Genetic Engineering: Genetically Modifying Organisms for Fun and Profit
Johan Sosa introduces foundational genetic engineering techniques, explaining how community biohackers modify bacteria to express fluorescent proteins.
Summary
Introduction to Genetically Modified Organisms (GMOs)
Genetic engineering involves precisely introducing foreign recombinant DNA into host organisms to express novel enzymes, proteins, or biological functions.
Fundamentals of Gene Expression and Transformation
Johan Sosa explains promoters, ribosomal binding sites, coding sequences, and terminators required for stable protein expression in bacteria.
Hands-On Protocols for Expressing GFP Proteins
Sosa walks through culturing bacteria, introducing Green Fluorescent Protein (GFP) plasmids, and verifying fluorescence under ultraviolet illumination.
Applications in Brewing, Materials, and Art
The presentation explores practical community biohacking projects—spanning custom yeast strains for brewing to bio-materials and bio-art.
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